preimmune mouse serum Search Results


preimmune serum  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology preimmune serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Preimmune Serum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc03698794-66-24-30?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
preimmune serum - by Bioz Stars, 2026-08
96/100 stars
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preimmune serum  (Rockland Immunochemicals)
86
Rockland Immunochemicals preimmune serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Preimmune Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc03434557-203-7-12?v=Rockland+Immunochemicals
Average 86 stars, based on 1 article reviews
preimmune serum - by Bioz Stars, 2026-08
86/100 stars
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preimmune serum  (Novus Biologicals)
93
Novus Biologicals preimmune serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Preimmune Serum, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc04706311-140-12-19?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
preimmune serum - by Bioz Stars, 2026-08
93/100 stars
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anti-scab polyclonal mouse serum  (Koma Biotech)
90
Koma Biotech anti-scab polyclonal mouse serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Anti Scab Polyclonal Mouse Serum, supplied by Koma Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pm33613493-81-3-14?v=Koma+Biotech
Average 90 stars, based on 1 article reviews
anti-scab polyclonal mouse serum - by Bioz Stars, 2026-08
90/100 stars
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normal mouse serum  (Rockland Immunochemicals)
94
Rockland Immunochemicals normal mouse serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Normal Mouse Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pm22407913-46-12-15?v=Rockland+Immunochemicals
Average 94 stars, based on 1 article reviews
normal mouse serum - by Bioz Stars, 2026-08
94/100 stars
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preimmune rabbit serum  (Cosmo Bio USA)
90
Cosmo Bio USA preimmune rabbit serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Preimmune Rabbit Serum, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pm09379508-41-6-9?v=Cosmo+Bio+USA
Average 90 stars, based on 1 article reviews
preimmune rabbit serum - by Bioz Stars, 2026-08
90/100 stars
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bovine serum  (Jackson Immuno)
93
Jackson Immuno bovine serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Bovine Serum, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pm11307168-256-15-17?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
bovine serum - by Bioz Stars, 2026-08
93/100 stars
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goat serum  (Jackson Immuno)
96
Jackson Immuno goat serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Goat Serum, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc08379704-67-5-38?v=Jackson+Immuno
Average 96 stars, based on 1 article reviews
goat serum - by Bioz Stars, 2026-08
96/100 stars
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anti-gbm sheep nephrotoxic serum  (Probetex Inc)
90
Probetex Inc anti-gbm sheep nephrotoxic serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Anti Gbm Sheep Nephrotoxic Serum, supplied by Probetex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc08164984-52-29-33?v=Probetex+Inc
Average 90 stars, based on 1 article reviews
anti-gbm sheep nephrotoxic serum - by Bioz Stars, 2026-08
90/100 stars
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polyclonal rabbit iggs  (SouthernBiotech)
90
SouthernBiotech polyclonal rabbit iggs
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Polyclonal Rabbit Iggs, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pm22407913-46-18-21?v=SouthernBiotech
Average 90 stars, based on 1 article reviews
polyclonal rabbit iggs - by Bioz Stars, 2026-08
90/100 stars
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rabbit anti c 6 serum  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit anti c 6 serum
Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or <t>preimmune</t> IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.
Rabbit Anti C 6 Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/preimmune+mouse+serum/pmc03620393-250-37-31?v=Rockland+Immunochemicals
Average 93 stars, based on 1 article reviews
rabbit anti c 6 serum - by Bioz Stars, 2026-08
93/100 stars
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Image Search Results


Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or preimmune IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: SMAD3 Deficiency Promotes Inflammatory Aortic Aneurysms in Angiotensin II–Infused Mice Via Activation of iNOS

doi: 10.1161/JAHA.113.000269

Figure Lengend Snippet: Molecular mechanism by which SMAD3 deficiency causes enhanced iNOS‐derived NO production in aorta. A and B, Fluorescence staining for NO, iNOS, and F4/80 in vehicle‐ (A) and AngII‐infused (B) WT and S3KO aortas. Dotted lines demarcate adventitial layer from medial layer. Scale bars=50 μm. C, Western blots showing increased C/EBPβ, phosphorylated NF‐κB, and iNOS in S3KO aorta. Beta‐tubulin served as a loading control. D, In vivo ChIP of C/EBP binding site within the mouse iNOS promoter and a control region 2 kb upstream of the C/EBP site using antibodies against C/EBPβ or preimmune IgG. The gene fragments in the immunoprecipitated chromatin were quantified by RT‐qPCR. Aliquots of the chromatin were also analyzed before immunoprecipitation (input). E, Re‐ChIP was performed using antibodies against coactivator p300. AngII indicates angiotensin II; iNOS indicates inducible nitric oxide synthase; WT, wild type; NO, nitric oxide; S3KO, SMAD3 knockout; C/EBP, CCAAT/enhancer binding protein; L, lumen; m, tunica media; a, tunica adventitia; NF‐κB, nuclear factor‐kappaB.

Article Snippet: Antibodies against CCAAT/enhancer binding protein (C/EBP)–β (#sc‐150), p300 (#sc‐585), α‐smooth muscle actin (α‐SMA [#sc‐69972]), β‐tubulin (#sc‐9104), horseradish peroxidase (HRP)–conjugated goat anti‐mouse IgG (#sc‐2005), and preimmune serum (#sc‐2338) were purchased from Santa Cruz Biotechnology.

Techniques: Derivative Assay, Fluorescence, Staining, Western Blot, Control, In Vivo, Binding Assay, Immunoprecipitation, Quantitative RT-PCR, Knock-Out